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Characterization of the genes involved in nitrogen cycling in wastewater treatment plants using DNA microarray

Junqin PANG, Masami MATSUDA, Masashi KURODA, Daisuke INOUE, Kazunari SEI, Kei NISHIDA, Michihiko IKE

《环境科学与工程前沿(英文)》 2016年 第10卷 第4期 doi: 10.1007/s11783-016-0846-x

摘要: Nitrogen-cycling microbial communities in municipal WWTPs were characterized. Numbers of , and genes were quantified by MPN-PCR. Diversity of whole nitrogen-cycling communities was analyzed with DNA microarray. CAS process retained diverse nitrogen cycling populations. Specific, limited populations may be dominated in nitrogen removal processes. To improve nitrogen removal performance of wastewater treatment plants (WWTPs), it is essential to understand the behavior of nitrogen cycling communities, which comprise various microorganisms. This study characterized the quantity and diversity of nitrogen cycling genes in various processes of municipal WWTPs by employing two molecular-based methods:most probable number-polymerase chain reaction (MPN-PCR) and DNA microarray. MPN-PCR analysis revealed that gene quantities were not statistically different among processes, suggesting that conventional activated sludge processes (CAS) are similar to nitrogen removal processes in their ability to retain an adequate population of nitrogen cycling microorganisms. Furthermore, most processes in the WWTPs that were researched shared a pattern:the and the bacterial genes were more abundant than the and archaeal genes, respectively. DNA microarray analysis revealed that several kinds of nitrification and denitrification genes were detected in both CAS and anaerobic-oxic processes (AO), whereas limited genes were detected in nitrogen removal processes. Results of this study suggest that CAS maintains a diverse community of nitrogen cycling microorganisms; moreover, the microbial communities in nitrogen removal processes may be specific.

关键词: DNA microarray analysis     Nitrogen cycling functional genes     Most probable number-polymerase chain reaction (MPN-PCR)     Wastewater treatment plants (WWTPs)    

SOIL NITROGEN CYCLING AND ENVIRONMENTAL IMPACTS IN THE SUBTROPICAL HILLY REGION OF CHINA: EVIDENCE FROM

《农业科学与工程前沿(英文)》 2022年 第9卷 第3期   页码 407-424 doi: 10.15302/J-FASE-2022448

摘要:

● Soil nitrogen fluxes and influencing factors were reviewed in the subtropical hilly regions.

关键词: nitrogen cycling     soil nitrogen     nitrogen deposition     greenhouse gases emission     non-point source pollution     nitrogen use efficiency    

Microbial community and functional genes in the rhizosphere of alfalfa in crude oil-contaminated soil

Yi ZHONG, Jian WANG, Yizhi SONG, Yuting LIANG, Guanghe LI

《环境科学与工程前沿(英文)》 2012年 第6卷 第6期   页码 797-805 doi: 10.1007/s11783-012-0405-z

摘要: A rhizobox system constructed with crude oil-contaminated soil was vegetated with alfalfa ( L.) to evaluate the rhizosphere effects on the soil microbial population and functional structure, and to explore the potential mechanisms by which plants enhance the removal of crude oil in soil. During the 80-day experiment, 31.6% of oil was removed from the adjacent rhizosphere (AR); this value was 27% and 53% higher than the percentage of oil removed from the far rhizosphere (FR) and from the non-rhizosphere (NR), respectively. The populations of heterotrophic bacteria and hydrocarbon-degrading bacteria were higher in the AR and FR than in the NR. However, the removal rate of crude oil was positively correlated with the proportion of hydrocarbon-degrading bacteria in the rhizosphere. In total, 796, 731, and 379 functional genes were detected by microarray in the AR, FR, and NR, respectively. Higher proportions of functional genes related to carbon degradation and organic remediation, were found in rhizosphere soil compared with NR soil, suggesting that the rhizosphere selectively increased the abundance of these specific functional genes. The increase in water-holding capacity and decrease in pH as well as salinity of the soil all followed the order of AR>FR>NR. Canonical component analysis showed that salinity was the most important environmental factor influencing the microbial functional structure in the rhizosphere and that salinity was negatively correlated with the abundance of carbon and organic degradation genes.

关键词: crude oil-contaminated soil     phytoremediation     rhizosphere effects     rhizobox     functional genes    

Overlooked nitrogen-cycling microorganisms in biological wastewater treatment

《环境科学与工程前沿(英文)》 2021年 第15卷 第6期 doi: 10.1007/s11783-021-1426-2

摘要:

• AOA and comammox bacteria can be more abundant and active than AOB/NOB at WWTPs.

关键词: Ammonia oxidizing archaea (AOA)     Complete ammonia oxidizing (comammox) bacteria     Dissimilatory nitrate reduction to ammonium (DNRA) bacteria     Nitrate/nitrite-dependent anaerobic methane oxidizing (NOx-DAMO) microorganisms     Engineering application    

ROOT EXUDATES FROM CANOLA EXHIBIT BIOLOGICAL NITRIFICATION INHIBITION AND ARE EFFECTIVE IN INHIBITING AMMONIA OXIDATION IN SOIL

《农业科学与工程前沿(英文)》 2022年 第9卷 第2期   页码 177-186 doi: 10.15302/J-FASE-2021421

摘要:

A range of plant species produce root exudates that inhibit ammonia-oxidizing microorganisms. This biological nitrification inhibition (BNI) capacity can decrease N loss and increase N uptake from the rhizosphere. This study sought evidence for the existence and magnitude of BNI capacity in canola ( Brassica napus). Seedlings of three canola cultivars, Brachiaria humidicola(BNI positive) and wheat ( Triticum aestivum) were grown in a hydroponic system. Root exudates were collected and their inhibition of the ammonia oxidizing bacterium, Nitrosospira multiformis, was tested. Subsequent pot experiments were used to test the inhibition of native nitrifying communities in soil. Root exudates from canola significantly reduced nitrification rates of both N. multiformis cultures and native soil microbial communities. The level of nitrification inhibition across the three cultivars was similar to the well-studied high-BNI species B. humidicola. BNI capacity of canola may have implications for the N dynamics in farming systems and the N uptake efficiency of crops in rotational farming systems. By reducing nitrification rates canola crops may decrease N losses, increase plant N uptake and encourage microbial N immobilization and subsequently increase the pool of organic N that is available for mineralization during the following cereal crops.

关键词: ammonia oxidizing microorganisms / biological nitrification inhibition / farming rotations / nitrogen cycling / nitrogen use efficiency    

Immunotherapeutic effects on murine pancreatic carcinoma by β-elemene combined with dendritic cells modified with genes

TAN Guang, WANG Zhongyu, CHE Luanqing, YIN Shuo

《医学前沿(英文)》 2007年 第1卷 第1期   页码 41-45 doi: 10.1007/s11684-007-0008-4

摘要: The dendritic cell vaccine is a treatment vaccine with potent clinical applications. Functional cytokines can enhance dendritic cell anti-tumor immune responses. This experiment was conducted to study the effects of bone marrow-deriv

关键词: Functional     experiment     anti-tumor     dendritic     clinical    

AGRONOMIC AND ENVIRONMENTAL BENEFITS OF REINTRODUCING HERB- AND LEGUME-RICH MULTISPECIES LEYS INTO ARABLE ROTATIONS: A REVIEW

《农业科学与工程前沿(英文)》 2022年 第9卷 第2期   页码 245-271 doi: 10.15302/J-FASE-2021439

摘要:

Agricultural intensification and the subsequent decline of mixed farming systems has led to an increase in continuous cropping with only a few fallow or break years, undermining global soil health. Arable-ley rotations incorporating temporary pastures (leys) lasting 1–4 years may alleviate soil degradation by building soil fertility and improving soil structure. However, the majority of previous research on arable-ley rotations has utilized either grass or grass-clover leys within ungrazed systems. Multispecies leys, containing a mix of grasses, legumes, and herbs, are rapidly gaining popularity due to their promotion in agri-environment schemes and potential to deliver greater ecosystem services than conventional grass or grass-clover leys. Livestock grazing in arable-ley rotations may increase the economic resilience of these systems, despite limited research of the effects of multispecies leys on ruminant health and greenhouse gas emissions. This review aims to evaluate previous research on multispecies leys, highlighting areas for future research and the potential benefits and disbenefits on soil quality and livestock productivity. The botanical composition of multispecies leys is crucial, as legumes, deep rooted perennial plants (e.g., Onobrychis viciifolia and Cichorium intybus) and herbs (e.g., Plantago lanceolata) can increase soil carbon, improve soil structure, reduce nitrogen fertilizer requirements, and promote the recovery of soil fauna (e.g., earthworms) in degraded arable soils while delivering additional environmental benefits (e.g., biological nitrification inhibition and enteric methane reduction). Multispecies leys have the potential to deliver biologically driven regenerative agriculture, but more long-term research is needed to underpin evidence-based policy and farmer guidance.

关键词: bioactive forages / integrated crop-livestock systems / nitrogen cycling / plant secondary metabolites / soil carbon / soil quality    

MANURE AND METROPOLES---A GLOBAL FRAMEWORK FOR NUTRIENT CYCLING IN FOOD SYSTEMS

《农业科学与工程前沿(英文)》 2021年 第8卷 第1期

Distribution of phytoplankton community and its influence factors in an urban river network, East China

Ling Sun, Hui Wang, Yuanqing Kan, Shiliang Wang

《环境科学与工程前沿(英文)》 2018年 第12卷 第6期 doi: 10.1007/s11783-018-1062-7

摘要:

Effects of urban river conditions on phytoplankton community were investigated.

Nitrate utilization and hydrodynamic condition affected phytoplankton distribution.

A winter bloom was induced by abundant ammonia, total phosphorus and organic matters.

The diatoms and euglenoids dominated in an urban beheaded river ecosystem.

关键词: Phytoplankton composition     Spatiotemporal distribution     Functional groups     Nitrate nitrogen     Winter bloom    

Metagenomic analysis on resistance genes in water and microplastics from a mariculture system

《环境科学与工程前沿(英文)》 2022年 第16卷 第1期   页码 4-4 doi: 10.1007/s11783-021-1438-y

摘要:

Microplastics existing widely in different matrices have been regarded as a reservoir for emerging contaminants. Mariculture systems have been observed to host microplastics and antibiotic resistance genes (ARGs). However, more information on proliferation of ARGs and metal resistance genes (MRGs) in mariculture system at the presence of microplastics is needed.

关键词: Antibiotic resistance genes     Metal resistance genes     Metagenomic analysis     Microplastics     Mariculture    

Elastic modulus and thermal stress in coating during heat cycling with different substrate shapes

Daniel GAONA,Alfredo VALAREZO

《机械工程前沿(英文)》 2015年 第10卷 第3期   页码 294-300 doi: 10.1007/s11465-015-0351-0

摘要:

The elastic modulus of a deposit (Ed) can be obtained by monitoring the temperature (?T) and curvature (?k) of a one-side coated long plate, namely, a one-dimensional (1D) deformation model. The aim of this research is to design an experimental setup that proves whether a 1D deformation model can be scaled for complex geometries. The setup includes a laser displacement sensor mounted on a robotic arm capable of scanning a specimen surface and measuring its deformation. The reproducibility of the results is verified by comparing the present results with Stony Brook University Laboratory’s results. The ?k-?T slope error is less than 8%, and the Ed estimation error is close to 2%. These values reveal the repeatability of the experiments. Several samples fabricated with aluminum as the substrate and 100MXC nanowire (Fe and Cr alloy) as the deposit are analyzed and compared with those in finite element (FE) simulations. The linear elastic behavior of 1D (flat long plate) and 2D (squared plate) specimens during heating/cooling cycles is demonstrated by the high linearity of all ?k-?T curves (over 97%). The Ed values are approximately equal for 1D and 2D analyses, with a median of 96 GPa and standard deviation of 2 GPa. The correspondence between the experimental and simulated results for the 1D and 2D specimens reveals that deformation and thermal stress in coated specimens can be predicted regardless of specimen geometry through FE modeling and by using the experimental value of Ed. An example of a turbine-blade-shaped substrate is presented to validate the approach.

关键词: in-plane     Young’s modulus     curvature temperature     thermal stress     coating    

MANURE AND METROPOLES—A GLOBAL FRAMEWORK FOR NUTRIENT CYCLING IN FOOD SYSTEMS

Daan VERSTAND, Theun VELLINGA, Krijn POPPE, Pieter de WOLF

《农业科学与工程前沿(英文)》 2021年 第8卷 第1期   页码 182-187 doi: 10.15302/J-FASE-2020364

Distribution and removal of antibiotic resistance genes during anaerobic sludge digestion with alkaline

Mengli Wang, Ruying Li, Qing Zhao

《环境科学与工程前沿(英文)》 2019年 第13卷 第3期 doi: 10.1007/s11783-019-1127-2

摘要:

Sludge digestion is critical to control the spread of ARGs from wastewater to soil.

Fate of ARGs in three pretreatment-AD processes was investigated.

UP was more efficient for ARGs removal than AP and THP in pretreatment-AD process.

The total ARGs concentration showed significant correlation with 16S rRNA gene.

The bacteria carrying ARGs could be mainly affiliated with Proteobacteria.

关键词: Tetracycline resistance genes     Sulfonamide resistance genes     16S rRNA gene     Sludge pretreatment     Anaerobic sludge digestion    

Environmental effects and risk control of antibiotic resistance genes in the organic solid waste aerobic

《环境科学与工程前沿(英文)》 2021年 第15卷 第6期 doi: 10.1007/s11783-021-1415-5

摘要:

• ARGs were detected in livestock manure, sludge, food waste and fermentation dregs.

关键词: Antibiotic resistance genes     Organic solid waste     Aerobic composting     Livestock manure     Sludge    

ultraviolet/chlorine process on microbial community structure, typical pathogens, and antibiotic resistance genes

《环境科学与工程前沿(英文)》 2022年 第16卷 第8期 doi: 10.1007/s11783-022-1521-z

摘要:

• UV/chlorine can effectively remove VBNC pathogens, ARGs and MGEs in reclaimed water.

关键词: UV/chlorine process     Pathogen     Antibiotic resistance genes     High-throughput qPCR     Reclaimed water    

标题 作者 时间 类型 操作

Characterization of the genes involved in nitrogen cycling in wastewater treatment plants using DNA microarray

Junqin PANG, Masami MATSUDA, Masashi KURODA, Daisuke INOUE, Kazunari SEI, Kei NISHIDA, Michihiko IKE

期刊论文

SOIL NITROGEN CYCLING AND ENVIRONMENTAL IMPACTS IN THE SUBTROPICAL HILLY REGION OF CHINA: EVIDENCE FROM

期刊论文

Microbial community and functional genes in the rhizosphere of alfalfa in crude oil-contaminated soil

Yi ZHONG, Jian WANG, Yizhi SONG, Yuting LIANG, Guanghe LI

期刊论文

Overlooked nitrogen-cycling microorganisms in biological wastewater treatment

期刊论文

ROOT EXUDATES FROM CANOLA EXHIBIT BIOLOGICAL NITRIFICATION INHIBITION AND ARE EFFECTIVE IN INHIBITING AMMONIA OXIDATION IN SOIL

期刊论文

Immunotherapeutic effects on murine pancreatic carcinoma by β-elemene combined with dendritic cells modified with genes

TAN Guang, WANG Zhongyu, CHE Luanqing, YIN Shuo

期刊论文

AGRONOMIC AND ENVIRONMENTAL BENEFITS OF REINTRODUCING HERB- AND LEGUME-RICH MULTISPECIES LEYS INTO ARABLE ROTATIONS: A REVIEW

期刊论文

MANURE AND METROPOLES---A GLOBAL FRAMEWORK FOR NUTRIENT CYCLING IN FOOD SYSTEMS

期刊论文

Distribution of phytoplankton community and its influence factors in an urban river network, East China

Ling Sun, Hui Wang, Yuanqing Kan, Shiliang Wang

期刊论文

Metagenomic analysis on resistance genes in water and microplastics from a mariculture system

期刊论文

Elastic modulus and thermal stress in coating during heat cycling with different substrate shapes

Daniel GAONA,Alfredo VALAREZO

期刊论文

MANURE AND METROPOLES—A GLOBAL FRAMEWORK FOR NUTRIENT CYCLING IN FOOD SYSTEMS

Daan VERSTAND, Theun VELLINGA, Krijn POPPE, Pieter de WOLF

期刊论文

Distribution and removal of antibiotic resistance genes during anaerobic sludge digestion with alkaline

Mengli Wang, Ruying Li, Qing Zhao

期刊论文

Environmental effects and risk control of antibiotic resistance genes in the organic solid waste aerobic

期刊论文

ultraviolet/chlorine process on microbial community structure, typical pathogens, and antibiotic resistance genes

期刊论文